High-Precision Analysis of Translational Pausing by Ribosome Profiling in Bacteria Lacking EFP
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چکیده
منابع مشابه
High-precision analysis of translational pausing by ribosome profiling in bacteria lacking EFP.
Ribosome profiling is a powerful method for globally assessing the activity of ribosomes in a cell. Despite its application in many organisms, ribosome profiling studies in bacteria have struggled to obtain the resolution necessary to precisely define translational pauses. Here, we report improvements that yield much higher resolution in E. coli profiling data, enabling us to more accurately as...
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Zupanic A, Meplan C, Grellscheid SN, Mathers JC, Kirkwood TBL, Hesketh JE, Shanley DP Centre for Integrated Systems Biology of Ageing & Nutrition, Institute for Ageing and Health, Newcastle University, Newcastle-upon-Tyne, NE4 5PL, UK Institute for Cell and Molecular Biosciences and Human Nutrition Research Centre, Newcastle University, Newcastle-upon-Tyne, NE2 4HH, UK School of Biological and ...
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Ribosomes translate genetic information into polypeptides in several basic steps: initiation, elongation, termination and recycling. When ribosomes are arrested during elongation or termination, the cell's capacity for protein synthesis is reduced. There are numerous quality control systems in place to distinguish between paused ribosomes that need some extra input to proceed and terminally sta...
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The formation of skeletal muscles is associated with drastic changes in protein requirements known to be safeguarded by tight control of gene transcription and mRNA processing. The contribution of regulation of mRNA translation during myogenesis has not been studied so far. We monitored translation during myogenic differentiation of C2C12 myoblasts, using a simplified protocol for ribosome foot...
متن کاملDetecting translational regulation by change point analysis of ribosome profiling data sets.
Ribo-Seq maps the location of translating ribosomes on mature mRNA transcripts. While during normal translation, ribosome density is constant along the length of the mRNA coding region, this can be altered in response to translational regulatory events. In the present study, we developed a method to detect translational regulation of individual mRNAs from their ribosome profiles, utilizing chan...
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ژورنال
عنوان ژورنال: Cell Reports
سال: 2015
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2015.03.014